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    DSS潰瘍性結腸炎造模-豬模型

    背景概述


    腸道通透性增加和相關體重減輕是DSS結腸炎動物模型的常?特征。D-甘露醇是?種惰性小碳水化合物分?,可沿整個隱窩-軸吸收,?于評估體內腸道通透性。

    1663578086242310.png 

    圖源百度


    實驗動物

    4-5天大,約克郡小豬

    實驗材料

    DSS(yeasen DSS 60316ES,MW36000-50000)

    實驗步驟

    1. 以商業代乳粉配?飲?,每天喂食三次,直到實驗結束;

    2. 將動物分為陽性對照組(Pos),陰性對照組(Neg),實驗組(Trp);

    3. 陽性對照組(Pos):灌注DSS;陰性對照組(Neg):灌注生理鹽水;實驗組(Trp):灌注DSS+治療藥物;

    4. 每只小豬每日攝入量1.25g DSS/kg,灌注5天。

    實驗結果



    1663578193907808.png 

    1. 腸道通透性分析

    DSS誘導致小豬體內D-甘露醇濃度升高,腸道屏障被破壞。

    1663578216471123.png 

    2. HE病理染色切片(A為Neg組;B為Pos組)


    DSS處理的Pos組
    隱窩結構扭曲、炎癥細胞浸潤到粘膜和粘膜下層、隱窩膿腫和隱窩炎。

    1663578246943424.png 

    3. 炎癥因子檢測

    DSS處理的Pos組中兩種細胞因?的濃度均升高[1]。

     

    翌圣DSS產品優勢

    翌圣提供高品質DSS(Cat.NO:60316ES,MW:36000~50000),具有以下優勢:

    1、高純度98%),硫含量17-19%,游離硫<0.2%,大量數據文獻支持,廣泛應用于UC模型的構建。

    2、多種屬動物中均可造模:小鼠、大鼠、斑馬魚、豬、果蠅;

    3、癥狀表現與人UC極度相似:可用于研究急、慢性結腸炎的發生發展機制,也可用于藥物的藥效研究。

    4、簡單易行,成模率高,重復性強

    5、聯合氧化偶氮甲烷 (azoxymethane, AOM)用藥,可用于誘發結腸炎相關性癌癥 (colitis associated cancer, CAC) 動物模型,成功模擬IBD誘發CAC的過程。

    1663578309396476.png

    建模效率與進口產品一致,產品價格低至進口價格的1/3,大量現貨庫存。


    產品訂購


    產品名稱

    貨號

    規格

    價格(元)

    促銷價(元)

    Dextran Sulfate Sodium Salt(DSS)

    結腸炎建模用葡聚糖硫酸鈉鹽 MW:36000~50000

    60316ES25

    25 g

    1255.00

    955.00

    Dextran Sulfate Sodium Salt(DSS)

    結腸炎建模用葡聚糖硫酸鈉鹽 MW:36000~50000

    60316ES60

    100 g

    3855.00

    2655.00

    Dextran Sulfate Sodium Salt(DSS)

    結腸炎建模用葡聚糖硫酸鈉鹽 MW:36000~50000

    60316ES76

    500 g

    16855.00

    9655.00

    Dextran Sulfate Sodium Salt(DSS)

    結腸炎建模用葡聚糖硫酸鈉鹽 MW:36000~50000

    60316ES80

    1 kg

    32855.00

    18655.00

     


    產品已發表文章(部分)

    向下滑動查看

    2022

    [1] Lujuan Xing, Lijuan Fu, Songmin Cao,et al.The Anti-Inflammatory Effect of Bovine Bone-Gelatin-Derived Peptides in LPS-Induced RAW264.7 Macrophages Cells and Dextran Sulfate Sodium-Induced C57BL/6 Mice[J]. Nutrients 2022, 14, 1479. IF=5.717

    [2] Lei-NingChen,TaoJing,Zi-BinLin,et al.Metabolomic and transcriptomic responses of mouse testis to the dextran sulfate sodium induced colitis[J].Reproductive Toxicology.2022, Pages 35-42.IF=3.143

    [3] YuangengLi,PingYu,WenwenFu,et al.Polysaccharides from Panax ginseng C. A. Meyer alleviated DSS-induced IBD by inhibiting JAK2/STAT1/NLPR3 inflammasome signalling pathway in mice[J].Journal of Functional Foods.2022, 105013. IF=4.451

    [4] Wang S,  Huang J,  Tan KS, et al.Isosteviol Sodium Ameliorates Dextran Sodium Sulfate-Induced Chronic Colitis through the Regulation of Metabolic Profiling, Macrophage Polarization, and NF-B Pathway[J].Oxidative Medicine and Cellular Longevity. 2022,4636618. IF=5.076

    [5] Chenglu Sun, Jingpeng Hao,Hong Qin,et al.Endometrial Regenerative Cell-Derived Conditioned Medium Alleviates Experimental Colitis.Stem Cells Int 2022;2022 IF=3.869

    2021

    [1] Li Zhao, Fei Wang,Zhengwei Cai,et al.Improving drug utilization platform with injectable mucoadhesive hydrogel for treating ulcerative colitis[J].chemical engineering journal.424(2021)130464.IF=16.744

    [2] Hao H,  Zhang X,  Tong L,  Liu Q,et al.Lactobacillus plantarumEffect of Extracellular Vesicles Derived From  Q7 on Gut Microbiota and Ulcerative Colitis in Mice[J].Frontiers in Immunology.2021.777147 .IF=7.561

    [3] Lingjun Tong, Xinyi Zhang , Haining Hao,et al.Lactobacillus rhamnosus GG Derived Extracellular Vesicles Modulate Gut Microbiota and Attenuate Inflammatory in DSS-Induced Colitis Mice[J].Nutrients.2021, 13, 3319. IF=5.717

    [4] Zhipeng Gu , Yujie Zhu, Fengfeng Mei,et al.Tilapia head glycolipids protect mice against dextran sulfate sodium-induced colitis by ameliorating the gut barrier and suppressing NF-kappa B signaling pathway[J].International Immunopharmacology.96 (2021) 107802. IF=3.943

    [5] JialiDong,YuanLi,HuiwenXiao,et al.Oral microbiota affects the efficacy and prognosis of radiotherapy for colorectal cancer in mouse models[J].Cell reports.2021, 109886.IF=9.423

    [6] Yaohua Fan,Yanqun Fan,Kunfeng Liu,et al.Edible Bird’s Nest Ameliorates Dextran Sulfate Sodium-Induced Ulcerative Colitis in C57BL/6J Mice by Restoring the Th17/Treg Cell Balance[J].Frontiers in Pharmacology.2021.632602.IF=7.561

    2020

    [1] Jia-Rong Huang, Sheng-Te Wang, Meng-Ning Wei,et al.Piperlongumine Alleviates Mouse Colitis and Colitis-Associated Colorectal Cancer[J].Frontiers in Pharmacology.2020.586885. IF=7.561

    [2] Gao X, Fan W, Tan L, et al. Soy isoflavones ameliorate experimental colitis by targeting ERα/NLRP3 inflammasome pathways[J]. The Journal of Nutritional Biochemistry, 2020, 83.IF=6.048

    [3] Li, Y., Dong, J., Xiao, H., Zhang, S., Wang, B., Cui, M., & Fan, S. Gut commensal derived-valeric acid protects against radiation injuries. Gut Microbes,.2020 .1–18.IF=10.245


    往期精彩

    1. 不同分子量葡聚糖硫酸鈉選購指南:高品質DSS葡聚糖硫酸鈉鹽

    2. 小鼠造模方法及具體步驟:Dextran Sulfate Sodium Salt(DSS)結腸炎造模解決方案 (yeasen.com)

    3. DSS結腸炎造模整體解決方案:Dextran Sulfate Sodium Salt(DSS) 潰瘍性結腸炎模型的建立 (yeasen.com)


    DSS(60316ES)參與發SCI贏獎勵活動,最高可得2000元禮品

    2022年 | 擴大獎勵范圍!Yeasen發SCI贏獎勵活動等你參與


    參考文獻

    [1] Connie J. Kim, et al. Journal of Nutritional Biochemistry 21 (2010) 468–475.

     

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